Submit genomes
Sign in to submit in batch; anonymous mode runs one small file for a quick test.
01Genome sequence (FASTA)
Drop FASTA files here, or click to choose
Anonymous: 1 file, up to 50 MB
02Gene annotation (GFF3)optional
Already have an NCBI / Prokka annotation (.gff / .gff3)? Attach it to rescue more candidate regions and refine boundaries. Leave empty otherwise.
Pick one FASTA file above first
03Analysis parametersdefaults work for most cases
Core thresholdStandard (recommended)0.95
How strict the model is when calling candidate regions. Higher = fewer, more confident calls; lower = more calls with possible false positives.
Extend threshold0.80
How far region boundaries may extend when edge evidence is weaker. Always capped by the core threshold.
Recommendation strictness
Stricter means fewer regions marked as "recommended for validation", with stronger evidence. Standard works for most cases.